Occurrence, Genotyping and Quantitative Microbial Risk Assessment of Cryptosporidium from Edible Bivalves of Manila Bay Philippines
The project focuses on carrying out hazard identification (detection of oocysts) and determining the occurrence of Cryptosporidium in bivalves from harvesting sites around Manila Bay using sucrose flotation and immunomagnetic separation (IMS) with immunofluorescence antibody assay (IFA) and PCR. The project also aims to determine the genotypes of Cryptosporidium oocysts obtained from the samples using DNA sequence and phylogenetic analyses. Furthermore, another goal of the project will be on assessing the risk of cryptosporidiosis on the healthy population of Metro Manila that regularly consumes these bivalves using Quantitative Microbial Risk Analysis.
Edison Jay A. Pagoso, M.Sc.